Ace2 binding kinetics


Performed using an Attana Cell™ 200 Biosensor. Expressed hACE2 at 50mg/ml (Expres2ion Biotechnologies) was immobilized on an Attana LNB carboxyl chip. The binding of S1 proteins to the chip was measured in a 2-fold serial dilution series (200 nM to 6.25 nM) in PBS, pH 7.4. Real-time binding (black curves) was fitted in a 1:1 simple binding model (red curves).

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